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Enterococcus Faecalis

RENGTY® Enterococcus faecalis (E.faecalis) is refined from natural microorganisms with advanced liquid fermentation and spray drying process, which is green environmental microbial multi-functional probiotic feed additives.

Key Words:

Enterococcus Faecalis powder

Enterococcus Faecalis feed grade

animal probiotics

E. Faecalis

lactobacillus

Category：

Pigment

E-mail:

susan.lee@rengty.com

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Enterococcus Faecalis

RENGTY^®Enterococcus faecalis (E.faecalis) is refined from natural microorganisms with advanced liquid fermentation and spray drying process, which is green environmental microbial multi-functional probiotic feed additives. It is one of the main flora in the intestinal tract of animals, which can produce lactic acid, vitamin, enzyme and natural antibiotics, beneficial to animal health. It is widely applied in feed additives of poultry, livestock, aquatic animals, ruminants and etc.

Specification

Specification	EF100	EF500	EF1000	EF5000
Viable count (CFU/g)	10 billion (1.0x1010)	50 billion (5.0x1010)	100 billion (1.0x1011)	500 billion (5.0x1011)
Color	White	Off-white	Light yellow	Yellow
Recommended dosage	100-200 g/t	20-40 g/t	10-20 g/t	2-4 g/t
Key component	Enterococcus faecalis, Carrier
Form	Powder
Odor	Slight fermentation odor
Particle size	More than 98% pass through standard sieve of 1.25mm
Loss on drying	≤10%
Total Arsenic (As)	≤2 mg/kg
Plumbum (Pb)	≤10 mg/kg
Fluorine (F)	≤1000 mg/kg
Mercury (Hg)	≤0.1 mg/kg
Cadmium (Cd)	≤0.5 mg/kg
Microbial contamination rate	≤0.5%
Coli group	≤1.0x105 CFU/g
Salmonella	None/25g
Total count of mold	≤2.0x107 CFU/g
Pathogenic bacteria	Negative

Biological feature
E.faecalis formerly classified as part of the Group D Streptococcus system-is a Gram-positive, commensal bacterium inhabiting the gastrointestinal tracts of humans and other mammals. It is a non-motile, facultatively anaerobic microbe. Most E.faecalis are round or oval, can extend along the chain direction, the diameter is 0.5-1.0um, most are line in double chain or short chain. This bacteria colony on the culture medium is big and smooth, the diameter is 1-2mm, whole reason, and pigment is rare. They require low nutrition; can live on the common nutrient agar medium. They can grow in the meat soup culture medium with the temperature of 10-45°C, PH value of 9.6 or containing 6.5 percent of NaCl, can bear the temperature of 65°C for 30 minutes, they can use arginine as energy, ferment sorbitol, do not ferment arabinose. They can grow on simple culture medium with folic acid.
Function

1. Effective prevention and treatment of diarrhea. It can protect the young animal against diarrhea, which are caused by disordering bacteria in intestinal tracts, and can adjust the disordering bacteria to normal level, decrease the usage of antibiotics.

Inhibit the reproduction of bacteria producing urease and putrefying bacteria in the intestinal tract and reduce the content of intestinal urease and endotoxin, decreases the content of ammonia and endotoxin in blood, lower concentrations of fecal ammonia, improve the breeding environment.

3. Enhance the animal's immune system function, improve the body disease resistance, prevent diseases, reduce digestive tract diseases and respiratory diseases. It can also intensify cellular and humoral immunity, reduce digestive tract diseases and respiratory diseases.

4. Inhibit the growth of E.coli, salmonella and staphylococcus aureus, improve the intestinal microenvironment. It’s a natural antimicrobial and antiseptic, is safe for animal and human.

5. It can synthesize a variety of vitamins and nutrients and provide nutrition for animal organism, promote growth, improve the production performance.

6. It can produce organic acids, adjust pH of intestinal tract, promote nutrient absorption.

7. It can activate the enzyme, promote nitrogen, sugar and lipid metabolism, improve the feed conversion rate and ADG, better meat quality, decrease the usage of enzyme.

Working Mechanism

E.faecalis can prevent pathogenic bacteria breeding by adhesion mechanism and competition exclusion, prevent disease. It can produce organic acid and volatile fatty acid, and fight nutritional ingredient with harmful bacteria to inhibit it’s growth, and that it can also form a biofilm to attach to the intestinal mucosa of animal to develop, produce and reproduce normally, form a lactobacillus barrier to resist the invasion of foreign toxins and pathogenic bacteria, and maintain the integrity of the intestinal epithelial barrier.

1. E.faecalis can produce metabolites and biological active substances to inhibit pathogenic microorganisms, consequently, establish and maintain the normal intestinal dominant population, reduces the adhesion ability of harmful bacteria, adjust intestinal microecosystem balance of animal.

2. E.faecalis can produce bacteriostatic substances such as bacteriocins and inhibit pathogenic bacteria such as escherichia coli and salmonella, and that it can also produce enterocin E5, which can effectively inhibit the growth and reproduction of listeria, staphylococcus aureus and putrefactive microorganisms, so it’s a natural antibiotics, beneficial to animal health.

3. E.faecalis can produce out rich L-lactic acid in the growth process, at the same time, it also can convert variety of carbohydrate anaerobic extract into lactic acid, completely absorbed and utilized by animal, which can lower pH of intestinal tract, promote absorption of vitamin D and minerals such as Ca, P and Fe.

4. E.faecalis can produce nonspecific immune adjustment factor, increase the degree of immunoglobulin and promote the production of antibodies, improve the body antibody levels, enhance the animal immune function.

5. E.faecalis can produce interferon, improve the activity of macrophage, promote the development of immune organs, improved animal immune response, strengthen the disease resistance.

6. E.faecalis can produce a variety of digestive enzymes, degrade part protein into amide and amino acid, soften the fibers in the feed, improve the utilization rate of feed. It also can promote amide and amino acid absorption, enhance the synthesis of tissue proteins and improve the digestion and absorption of nutrient substance.

Advantages

1. Better heat resistance and acid resistance than other lactobacillus.

Heat resistance: Residual activity is about 70% after heat-treatment in water bath for 3 min at 70℃

Acid resistance: Residual activity is above 97% after acid treatment for 2 hours at pH 2.0

2. Excellent acid-producing ability.

It can produce out vast lactic acid in it's growth process, 1x108 enterococcus faecalis product can produce out lactic acid 1.08g/L after growing 24 hours.

3. Good stability.

At indoor temperature, storage 6 months, 10 billion product residual activity is more than 93%, 20 billion product residual activity is more than 92%, 100 billion product residual activity is more than 90%

4.Strong stress resistance, easy to be resuscitated, reproduced and colonized

5.Safety, green and non-toxic without pollution and side effects.

Usage and dosage

This product is 100% natural feed additive, suitable for all animals such as pig, poultry, ruminant, aquatic animal and pet.The following dosage is an example of 10 billion cfu/g. Other specification can be converted according to the content of CFU.

Animal Species	Dosage (g/ton of feed)
Poultry	100
Pig	150
Weaning diarrhea	200-500
Aquatic Animal	150-200
Ruminant	150-200
Drinking water	160g/t water, one time per day
Purify water	50g/m2, soluble in water and directly splash, one time every 10-15 days

Application control：
Enterococcus faecalis belongs to enterococcus and has a strong acid producing ability. Compared with the core bacterial groups in the intestinal tract-bifidobacterium and lactobacillus acidophilus, enterococcus faecalis is more active. When it is used in creep feed, the dosage needs to be controlled to reduce the intestinal stress caused by the massive reproduction of enterococcus faecalis.
Packaging and delivery

Sample	20g/bag, 200g/bag, 300g/bag, 500g/bag, 1kg/bag, 2kg/bag, 5kg/bag
Packaging	20kg/25kg/bag, 20kg/25kg/drum or as requirements
Pallet	If 25kg/bag, 1000kg/40bag/pallet
Container	One 20'GP can contain 10-14 tons if bag
Port of loading	Qingdao/Shanghai/Tianjin or any port of China as requirements
Storage	Stored 12 months sealed in a cool, dry and airy place. Prevent from direct sunlight, heat and dampness, avoid stored with poisonous and harmful substances
Delivery time	10-20 days shipment after signed contract
Cautions	Fecal enterococcus does not form spore, can not be stored in damp environment, in summer at high temperature and wet, attention moisture proof. It is easy to absorb moisture, need to be sealed, as soon as possible use it after open the package.

By Express	By Air	By sea	By land
Suitable for under 50kg Fast: 3-7 days High cost Door to door service Easy to pick up the goods	Suitable for more than 50kg Fast: 3-7 days High cost Port to port service Professional broker needed	Suitable for more than 500kg Slow: 7-45 days Low cost Port to port service Profissional broker needed	Suitable for more than 500kg When by sea can not arrive at you, you can select land transportation, by train or by motor

General speaking, EMS, TNT, DHL, UPS by express, by air and by sea is main shipping terms.
By express for quantity less than 50kg cargo, also called DDU service
By sea for quantity over 500kg
By air is available for above 50kg
For high value products, please select air shipping or DHL express for safe
If the cargo quantity is more than 500kg, but by sea can not arrive at you, please select land transportation, by train or by motor.

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