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Water soluble bacillus subtilis

RENGTY® Water soluble bacillus subtilis is refined from natural microorganisms with advanced liquid fermentation and spray drying process, which is green environmental microbial multi-functional probiotic feed additives.

Key Words:

water soluble bacillus subtilis bacillus subtilis water soluble B. subtilis B. subtilis probiotics bacillus subtilis

Category：

Feed Enzymes

E-mail:

susan.lee@rengty.com

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Water soluble bacillus subtilis

RENGTY^® Water soluble bacillus subtilis is refined from natural microorganisms with advanced liquid fermentation and spray drying process, which is green environmental microbial multi-functional probiotic feed additives. It is avirulent, harmless, no residues, no pollution and the effect is remarkable, which is the best choice for green healthy and environment friendly breeding. It is widely applied in feed additives of poultry, livestock, aquatic animals, ruminants and etc.

Specification

Specification	WBS200	WBS500	WBS1000
Viable count (CFU/g)	20 billion (2.0x1010)	50 billion (5.0x1010)	100 billion (1.0x1011)
Color	Off-white	Light yellow	Yellow
Recommended dosage	100-200 g/t	40-80 g/t	20-40 g/t
Key component	Bacillus subtilis, Water soluble carrier
Form	Powder
Odor	Slight fermentation odor
Particle size	More than 98% pass through standard sieve of 60 meshes (0.25mm)
CAS No.:	68038-70-0
Loss on drying	≤10%
Total Arsenic (As)	≤2 mg/kg
Plumbum (Pb)	≤20 mg/kg
Fluorine (F)	≤1000 mg/kg
Mercury (Hg)	≤0.1 mg/kg
Cadmium (Cd)	≤0.5 mg/kg
Microbial contamination rate	≤1.0%
Coli group	≤1.0x105 CFU/g
Salmonella	None/25g
Total count of mold	≤2.0x107 CFU/g
Pathogenic bacteria	Negative

Biological feature
Bacillus subtilis, known also as the hay bacillus or grass bacillus, it’s a member of bacillus, gram positive bacteria. One bacterium is 0.7-0.8um×2-3um, coloring evenly, without capsule, with flagella around it, can move. The size of spore is 0.6-0.9um×1.0-1.5um, oval or columnar, at central or a little near center of the bacterium; the bacterium will not expand after spore forms. The surface of the bacteria colony is rough and opaque, with the color of dirty white or yellow, usually form wrinkle pellicle during growing in the liquid culture medium. They are aerobic bacteria, can use protein, a variety of sugar and starch decompose tryptophan and form benzpyrole.
B. subtilis is rod-shaped, and has the ability to form a tough, protective endospore, allowing the organism to tolerate extreme environmental conditions
Function

1. Adjust effectively micro-ecology bacteria group balance in animal gut, restrain the growth of colibacillus, salmonella and other harmful bacteria and promote the growth of beneficial bacteria breeding, such as lactobacillus, bifidobacterium and etc.

2. It can control bacterial disease, protect against the gastrointestinal diseases like diarrhea etc.. reduce animal morbidity and the dosage of antibiotic.

3. Increase digestion utilization rate of feed, promote animal growth, reduce feed dosage and improve the feed efficiency.

4. Reduce excretion of ammonia and nitrogen in animal waste, decrease the concentration of harmful gas in breeding place for livestock and poultry, remove bad smell and flying, improve the breeding condition and reduce environment pollution.

5. Inhibit harmful mircroorganisms, promote the digestion and absorption, increase feed using, prevent animal from indigestion.

6. As the food of the animals. The beneficial bacteria colonies are the good food for the animals, including the rich active nutrients to promote the growth.

7. It can improve the animals’ immunity, strengthen their constitution, increase the survival rate and growing speed, and enhance the anti-stress ability, promote animals’ health.

8. Purify water, remove the mud, adjust and steady the water PH value

Working Mechanism

This products compete with harmful bacterium for the attachment points in digestive tract, produce antibacterial material, and compete with harmful bacteria to get nutrients, strive for oxygen by biological mechanisms ,thereby, establish normal microflora in digestive tract; adjust and improve immune function; improve digestion and absorption function; prevent toxic amine synthesized.

1.The active substances like subtilin, polymyxin, nystatin, gramicidin and etc. Generated during the growing period of bacillus subtilis, have obvious inhibiting function to pathogenic bacteria.

2. They can exhaust the free oxygen in the environment rapidly, make the intestinal tract hypoxia, promote the beneficial anaerobic bacteria growing, and generate the organic acid like lactic acid and etc., reduce the PH value of intestinal tract, inhibit the growth of other pathogenic bacteria indirectly.

3. They can stimulate the growth and development of the immune organ of animals, activate lymphocyte T, B, lift the level of immune protein and antibody, enhance the functions of cell immunity and humor immunity, and enhance the immunity of the group.

4. The bacillus subtilis can compound α-amylase, protease, lipase, cellulase and etc. by themselves, and play a role with digestive enzymes inside animals in the digestive tract.

5. They can compound several kinds of vitamin group B like vitamin, niacin and etc., enhance the activity of interferon and macrophage inside animals.

Advantages

1. High spore content, stable performance, strong activity and long-lasting effect.

2. The consumption of free oxygen in the digestive tract and the creation of anaerobic environment are conducive to the growth of beneficial bacteria and the improvement of intestinal microecological environment.

3. Refined and extracted by deep ventilation liquid fermentation process with high content of CFU, and no miscellaneous bacteria.

4. Good stability, such as anti-pepsin, acid-proof, salt-tolerant, heat-resistant and compression resistant; maintain high stability during feed granulating and the process of getting though the acidic condition in stomach.

5. Produced a variety of strong activity available enzymes by metabolism including protease, lipase,α-amylase, cellulase etc., which can effectively complement endogenous enzymes in intestinal tract, thereby, improve digestion and absorption of animals, promote animal growth.

6. Produce B vitamins such as B1, B2, B6, vitamin C and vitamin K2 during the growth and breeding of animals, which provides vitamin nutrition for animals.

7. Generate subtilin, polymyxin, short bacilli peptide etc. active substances in the process of growth, which can obvious inhibit pathogenic bacteria or endogenous infection conditioned pathogenic bacteria. Replace part of antibiotics, it is safe, green, non-toxic, no residue, non-corrosive, no compatibility taboo without pollution and side effects, harmless to animals.

8. This product is easy to use. With biodegradablity and ecological security, it has no negative effects to environment.

Usage and dosage

This product is suitable for all animals such as pig, poultry, ruminant and aquatic animal.The following dosage is an example of 20 billion cfu/g. Other specification can be converted according to the content of CFU.

Animal Species	Dosage (g/ton of feed)
Piglet	200-300
Growing and fatting pig	100-200
Breeding/Egg Poultry	150-200
Meat Poultry	150-200
Ruminant	200-300
Aquatic Animal	300-500

For animal drinking water: in proportion 1:250 to 1:500 (for example, 1 Ton water need 2-4KG), wrapped with gauze and immersed in water for hours, then squeeze the gauze dry, finally add this liquid to animal drinking water.
Packaging and delivery

Sample	20g/bag, 200g/bag, 300g/bag, 500g/bag, 1kg/bag, 2kg/bag, 5kg/bag
Packaging	20kg/25kg/bag, 20kg/25kg/drum or as requirements
Pallet	If 25kg/bag, 1000kg/40bag/pallet
Container	One 20'GP can contain 10-14 tons if bag
Port of loading	Qingdao/Shanghai/Tianjin or any port of China as requirements
Storage	Stored 12 months sealed in a cool, dry and airy place. Prevent from direct sunlight, heat and dampness, avoid stored with poisonous and harmful substances
Delivery time	10-20 days shipment after signed contract
Precautions	Even though this product is non-toxic and biodegradable, it may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.

By Express	By Air	By sea	By land
Suitable for under 50kg Fast: 3-7 days High cost Door to door service Easy to pick up the goods	Suitable for more than 50kg Fast: 3-7 days High cost Port to port service Professional broker needed	Suitable for more than 500kg Slow: 7-45 days Low cost Port to port service Profissional broker needed	Suitable for more than 500kg When by sea can not arrive at you, you can select land transportation, by train or by motor

General speaking, EMS, TNT, DHL, UPS by express, by air and by sea is main shipping terms.
By express for quantity less than 50kg cargo, also called DDU service
By sea for quantity over 500kg
By air is available for above 50kg
For high value products, please select air shipping or DHL express for safe
If the cargo quantity is more than 500kg, but by sea can not arrive at you, please select land transportation, by train or by motor.

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